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A tripartite transcription factor network for PGC specification - NEW DATE

Þriðjudagur, April 23, 2013 -
12:30 to 13:10
Nánari staðsetning: 
Stofa 131

Erna Magnusdottir works at the Biomedical center at the University of Iceland. She recently completed a post doc at The University of Cambridge.

Her talk is titled  A tripartite transcription factor network for PGC specification.


Háskóli Íslands

Note - the talk has been moved to April 23rd - it will not be on Friday the 19th.

Abstract of the talk:

PGCs are the founder cells of the germ line and have the remarkable property of being able to epigenetically re-program the cellular genome, thus re-setting it for the generation of the totipotent zygote upon fertilization.

We have studied the transcriptional regulators Blimp1, AP2γ and Prdm14, all known to be crucial for the proper specification of PGCs in the mouse embryonic epiblast. Although the genetic evidence demonstrated their importance, the precise molecular mechanisms controlled by these factors have not been elucidated.

We performed genome wide location analysis for Blimp1 and AP2γ, and used their binding profiles together with profiles for Prdm14, to re-construct a transcriptional network for PGC specification. We demonstrated that the repression of somatic gene repression upon PGC specification is a more complex process than thought before, where all three factors play a role. In addition we show that Blimp1 directly binds AP2γ and induces it, where it subsequently works in conjunction with Prdm14 to induce the PGC transcriptional programme. We therefore conclude that this tripartite transcription factor network is sufficient to initiate the key events required for PGC specification.

List of other talks in the biology series is available the Institute of Life and Environmental Science.

Admission is free. Image copyright Erna Magnusdottir, shows binding of three transcription factors to a small region in the mouse genome.


References: Gillich A, Bao S, Grabole N, Hayashi K, Trotter MW, Pasque V, Magnúsdóttir E, Surani MA.Epiblast stem cell-based system reveals reprogramming synergy of germline factors. Cell Stem Cell. 2012 Apr 6;10(4):425-39. doi: 10.1016/j.stem.2012.01.020.

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